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Author: Kampf et al. (2011) Source: BMC Proc. 2011; 5(Suppl 6): P27


Kampf et al. (2011)

A novel method to determine the antiviral efficacy of hand rubs using murine norovirus (MNV) as surrogate of human norovirus

Background: Virucidal efficacy of hand disinfectants is determined in Europe by suspension tests. No method is yet available in Europe to determine the efficacy on contaminated hands. Therefore   the authors of the study aimed to develop a method which resembles both contamination and hand treatment in clinical practice as closely as possible.

Methods: Murine norovirus (MNV) was used as a test virus which is non pathogenic in humans but closely related to the human norovirus. Test persons contaminated their fingerpads in a MNV suspension supplemented with 5% stool. After air-drying of fingers, samples were taken by putting vials with sampling fluid onto the fingers followed by shaking. Fingerpads were recontaminated, followed by a 30 s treatment with 3 ml of hand rub or, as a control, with water. Four different hand rubs and one hand gel were tested. Sampling was then repeated and virus titers in sampling fluids were determined using cell culture.

Results: The ethanol-based products lead to mean log10-reductions of MNV titres of 4.32 (80% ethanol), 4.59 (85% ethanol, rinse), 4.52 (85% ethanol, gel) and 4.44 (95% ethanol) which were all significantly more effective compared to the application of water (means between 2.29 and 2.67). The propanol-based hand rub (30% propan-1-ol, 45% propan-2-ol and 0.2% mecetronium etilsulphate) was less effective (3.77).

Conclusion: The new method allows determining the efficacy of hand rubs under conditions reflecting clinical practices. The tested ethanol-based hand rubs lead to > 99.99 % reduction of MNV infectivity.

BMC Proc. 2011; 5(Suppl 6): P27

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