Research for infection protection

European Norms (EN)

The European Norms (EN) are European standards for testing disinfectants. The test methods are very much geared to practice and allow to compare disinfectants in Europe.

(Please click on image to enlarge.)

European Norms (EN)

Efficacy testing:

Testing of bactericidal activity, fungicidal activity and mycobactericidal activity:

  • Phase 1
    Quantitative suspension test, clean conditions, basic test.

    This is to test whether active ingredients of a chemical disinfectant or antiseptic are active for the areas described in the scope of application. Mycobacterial testing is not done in Phase 1.

    Additional note: If a product is tested in accordance with Phase 2, Phase 1 testing must not be carried out.

  • Phase 2
    This testing is divided into two steps:

    Step 1: Quantitative suspension test, dirty conditions
    This test’s objective is to test the spectrum of activity. The quantitative test demonstrates whether the product reacts with substances, such as proteins, and its activity is probably impaired.

    Step 2: Practical test
    Phase 2/Step 2 testing of hand and skin disinfectants is either performed by contaminating hands with test organisms or by determining the reduction of the skin flora compared to a reference substance.

    The quantitative tests vary depending on the disinfectant’s application area. The results define the claim of concentration and exposure time.

    In human medicine, Phase 2/Step 2 testing is only performed for hands and instruments at the moment.

For more details on the European Norms, please visit

The German listing is reviewed on the German Website of the BODE SCIENCE CENTER.