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Efficacy against Mycobacterium tuberculosis.
Mycobactericidal efficacy testing of disinfectants is carried out in stages:
Tuberculocidal activity: efficacy against M. tuberculosis (tested with M. terrae)
Mycobactericidal activity: efficacy against all mycobacteria
Standards require in vitro tests for proving the efficacy (quantitative suspension test; in the EN referred to as phase 2/step 1, e.g. according to EN 14348).
Test organism for the tuberculocidal spectrum of activity:
Mycobacterium terrae.
The methods of the European Norms (EN) and the German Society for Hygiene and Microbiology (DGHM) for testing disinfectants for their tuberculocidal efficacy are identical to a great extent.